流行性出血病病毒(EHDV)血清7型毒株在中国的首次分离与鉴定

doi:10.11843/j.issn.0366-6964.2019.03.015

Abstract

Abstract:

Epizootic haemorrhagic disease (EHD) is an infectious, non-contagious insect transmitted disease of ruminants. To investigate the infection of epizootic hemorrhagic disease virus (EHDV) among ruminants in Yunnan Province of China and study the virus genetic characteristics, monitoring points were set up in Shizong. Virus isolation was carried out through the inoculating BHK-21 cells with EHDV nucleic acid positive blood samples collected from the sentinel herds. Serotype of the isolated virus was identified by serum neutralization test; ORF of Seg-2 and Seg-3 of the virus were amplified with specific primers, then cloned and sequenced; antibody levels and viral nucleic acid in the EHDV infected animals throughout the year were tested by C-ELISA and qRT-PCR. Results were as follows:In August 2013, an EHDV strain (YNSZ/V269/2013) was isolated from Sentinel Cattle in Shizong, Yunnan. The serum neutralization test showed that the YNSZ/V269/2013 was an EHDV-7 virus. Sequence analysis of Seg-2 and Seg-3 showed that the isolated virus belonged to EHDV-7 Eastern type and had the closest genetic relationship with the Serotype 7 strain in Japan or Australia. The animal serum antibody increased rapidly after being infected by EHDV-7 and the antibody reached the highest point after 3 weeks and lasted for a longer time; meanwhile the nucleic acid content of the virus declined rapidly, 7 weeks later could not be detected in blood samples. This study reported the first isolation of EHDV serotype 7 in China and the virus sequence characterization of Seg-2 and Seg-3. The research results provide the basis for further development of the whole genome sequencing of Chinese EHDV-7 virus, epidemiological investigation, establishment of diagnostic methods and pathogenicity research.

CLC Number:

S852.659.4

YANG Zhenxing, MENG Jinxin, XIAO Lei, ZHU Jianbo, LIAO Defang, GAO Lin, LI Zhanhong, YANG Heng, LI Huachun. Isolation of Epizootic Haemorrhagic Disease Virus Serotype 7 Strain in China for the First Time[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 602-610.

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Isolation of Epizootic Haemorrhagic Disease Virus Serotype 7 Strain in China for the First Time

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